Development of 47 new microsatellite markers from a BTA6 library
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Updated: Sep 27, 2026
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
Published on: July 3, 2016
K M Reed1, N Ihara, F A Ponce de León
1Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN, USA.
Abstract:
Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15 and (GA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family.
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