Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transferring substrates to the 26S proteasome.

Rasmus Hartmann-Petersen1, Michael Seeger, Colin Gordon

  • 1August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK-2100 O, Copenhagen, Denmark.

Trends in Biochemical Sciences
|January 9, 2003
PubMed
Summary

Ubiquitin-dependent protein degradation recycles amino acids and controls regulatory proteins. Ubiquitin chains target proteins to the 26S proteasome, involving chaperones in substrate transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blocking protein quality control degradation leads to structural stabilization of DHFR indel variants.

The FEBS journal·2026
Same author

Complete genome sequence of <i>Rhodococcus erythropolis</i> strain ICBD2, isolated from hydrocarbon-polluted coastal sediments in Central Chile.

Microbiology resource announcements·2026
Same author

Seasonal Effects on Pathogenicity and Biocontrol Management of <i>Botryosphaeria</i> Dieback in <i>Vitis vinifera</i> L. cv. Cabernet Sauvignon and Sauvignon Blanc Under Field Conditions.

Plants (Basel, Switzerland)·2026
Same author

Proteasomal control of transcription factors: mechanisms, regulation and dysregulation.

Cellular and molecular life sciences : CMLS·2026
Same author

Copper resistance and genetic determinants in Chilean strains of Clavibacter michiganensis the causal agent of bacterial canker of tomato.

Pest management science·2026
Same author

A near-complete map of human cytosolic degrons and their relevance for disease.

Science advances·2026

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Ubiquitin-dependent protein degradation is crucial for cellular protein homeostasis.
  • The 26S proteasome complex degrades ubiquitinated proteins in eukaryotic cells.

Purpose of the Study:

  • To elucidate the molecular mechanisms of substrate transfer in the ubiquitin-proteasome pathway.
  • To understand the role of substrate-recruiting proteins and chaperones in this process.

Main Methods:

  • Literature review of recent reports on ubiquitin-proteasome pathway mechanisms.
  • Analysis of molecular interactions between ubiquitination machinery, substrate-recruiting proteins, chaperones, and the proteasome.

Main Results:

  • Clarification of molecular mechanisms for transferring ubiquitinated substrates to the proteasome.

Related Experiment Videos

  • Identification of direct or indirect transport routes involving substrate-recruiting proteins.
  • Evidence suggesting the involvement of chaperones in substrate transportation.

    • Conclusions:

    • The transport of ubiquitinated substrates to the proteasome is a critical, regulated step.
    • Substrate-recruiting proteins and chaperones play significant roles in facilitating this transport.
    • Further understanding of these mechanisms is key to comprehending protein degradation regulation.