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Rapid enterovirus RNA detection in clinical specimens by using nucleic acid sequence-based amplification
Marie L Landry1, Robin Garner, David Ferguson
1Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA. marie.landry@yale.edu
Journal of Clinical Microbiology
|January 9, 2003
Summary
Nucleic acid sequence-based amplification offers a rapid method for enterovirus (EV) detection, complementing traditional EV isolation in cell culture for enhanced diagnostic capabilities.
Area of Science:
- Virology
- Molecular Biology
- Diagnostic Microbiology
Background:
- Enteroviruses (EVs) are significant human pathogens.
- Accurate and rapid detection of EVs is crucial for clinical diagnosis and management.
- Traditional methods like cell culture isolation can be time-consuming.
Purpose of the Study:
- To compare the efficacy of nucleic acid sequence-based amplification (NASEA) with conventional cell culture for enterovirus detection.
- To evaluate the NucliSens Basic kit for RNA detection in enterovirus diagnostics.
Main Methods:
- Enterovirus detection was performed using nucleic acid sequence-based amplification (NASEA) with the NucliSens Basic kit.
- Virus isolation was conducted by inoculating samples into various cell lines, including MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and Buffalo green monkey kidney cells.
Main Results:
- NASEA provides a molecular-based approach for enterovirus detection.
- Cell culture methods involve the inoculation of clinical samples into a panel of susceptible cell lines.
- The study aimed to establish the performance characteristics of NASEA against cell culture.
Conclusions:
- NASEA represents a valuable molecular diagnostic tool for enterovirus detection.
- Comparison with cell culture aids in understanding the strengths and limitations of each method.
- This evaluation contributes to optimizing diagnostic workflows for enteroviral infections.