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Related Experiment Videos

Glucose utilization and recycling in ponies.

M S Anwer, T E Chapman, R Gronwall

    The American Journal of Physiology
    |January 1, 1976
    PubMed
    Summary

    Fasting significantly decreases glucose levels in ponies and alters glucose metabolism pathways. Recycling of glucose carbon becomes more significant during fasting, impacting overall glucose production.

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    Area of Science:

    • Equine metabolism
    • Glucose kinetics
    • Biochemical pathways

    Background:

    • Understanding glucose metabolism is crucial for equine health.
    • The impact of fasting on glucose recycling pathways in ponies requires further elucidation.
    • Previous studies have not fully characterized the contribution of different metabolic pathways to glucose production in fasted ponies.

    Purpose of the Study:

    • To compare glucose metabolism variables in fed and fasted ponies.
    • To quantify the relative recycling of glucose carbon in different metabolic states.
    • To determine the contribution of the Cori cycle and futile cycling to glucose production in ponies.

    Main Methods:

    • Utilized [U-14C]glucose and tritiated glucose ([6-T]glucose and [3-T]glucose) to trace glucose metabolism.
    • Compared glucose metabolism variables between fed and fasted pony groups.
    • Quantified relative recycling of glucose carbon and tritium.

    Main Results:

    • Fasting significantly reduced minimal mass of total-body glucose in ponies.
    • In fed ponies, recycling of glucose carbon was negligible for [6-T]glucose and [3-T]glucose studies but 16% for [2-T]glucose.
    • In fasted ponies, relative recycling was 12% for [6-T]glucose and 14% for [3-T]glucose studies.
    • The Cori cycle contributed negligibly to glucose production in fed ponies but 12% in ponies fasted for 72 hours.
    • A futile cycle at the glucose 6-phosphate stage accounted for 16% of plasma glucose carbon in fed ponies.

    Conclusions:

    • Fasting alters glucose metabolism in ponies, reducing total glucose mass and increasing carbon recycling.
    • [6-T]glucose and [3-T]glucose are reliable tracers for estimating glucose utilization in ponies.
    • Futile cycling and the Cori cycle play roles in glucose metabolism, particularly during fasting.

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