Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NESbase version 1.0: a database of nuclear export signals.

Tanja la Cour1, Ramneek Gupta, Kristoffer Rapacki

  • 1Center for Biological Sequence Analysis, Building-208, Technical University of Denmark, DK-2800 Lyngby, Denmark.

Nucleic Acids Research
|January 10, 2003
PubMed
Summary

NESbase is a new database detailing Leucine-rich Nuclear Export Signals (NES) critical for protein export. Surprisingly, most validated NES sequences do not match the common consensus pattern.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimorbidity trajectories and their sex-specific impacts on risk of mortality and re-hospitalisation.

Scientific reports·2026
Same author

Stability and interaction defects in the Sin3A-PAH1 αα-hub domain associated with loss-of-function variants.

The Journal of biological chemistry·2026
Same author

A biological-systems-based analysis using proteomic and metabolic network inference reveals mechanistic insights into hepatic steatosis.

Metabolism: clinical and experimental·2026
Same author

Predictors of Glycemic Response to Sulfonylurea Therapy in Type 2 Diabetes Over 12 Months: Comparative Analysis of Linear Regression and Machine Learning Models.

JMIR diabetes·2026
Same author

Large Language Model-informed ECG Dual Attention Network for Heart Failure Risk Prediction.

IEEE transactions on big data·2025
Same author

A Biological-Systems-Based Analyses Using Proteomic and Metabolic Network Inference Reveals Mechanistic Insights into Hepatic Lipid Accumulation: An IMI-DIRECT Study.

medRxiv : the preprint server for health sciences·2025

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Cell Biology

Background:

  • Nuclear export of proteins is crucial for cellular function.
  • Leucine-rich Nuclear Export Signals (NES) mediate this process.
  • Existing protein databases lack comprehensive NES annotations.

Purpose of the Study:

  • To create NESbase, a curated database of experimentally validated Leucine-rich NESs.
  • To provide detailed information on NES necessity, sufficiency, and CRM1 mediation.
  • To analyze NES sequence conservation and compare it to established consensus.

Main Methods:

  • Literature curation to identify and validate Leucine-rich NES sequences.
  • Compilation of data including export necessity, sufficiency, and CRM1 interaction.

Related Experiment Videos

  • Generation of a sequence logo to visualize amino acid conservation in NESs.

    • Main Results:

    • NESbase successfully compiles experimentally validated Leucine-rich NESs.
    • Information on export mechanism and receptor involvement (CRM1) is included.
    • Analysis revealed only 36% of validated NESs conform to the canonical L-x(2,3)-[LIVFM]-x(2,3)-L-x-[LI] consensus.

    Conclusions:

    • NESbase serves as a valuable resource for researchers studying nuclear export.
    • The findings challenge the universality of the widely accepted NES consensus sequence.
    • Further research into NES diversity and recognition mechanisms is warranted.