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Updated: Jul 29, 2026
13:47
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
REBASE: restriction enzymes and methyltransferases.
Richard J Roberts1, Tamas Vincze, Janos Posfai
1New England BioLabs, Inc., 32 Tozer Road, Beverly, MA 01915, USA. roberts@neb.com
Nucleic Acids Research
|January 10, 2003
Summary
REBASE is a comprehensive database for restriction enzymes and DNA methyltransferases, offering detailed data on recognition sites, isoschizomers, and methylation sensitivity. It also includes information on restriction-modification systems in bacterial and archaeal genomes.
Area of Science:
- Molecular Biology
- Genomics
- Bioinformatics
Background:
- Restriction enzymes and DNA methyltransferases are crucial tools in molecular biology.
- Understanding restriction-modification (R-M) systems is vital for genome analysis and biotechnology.
- Databases are essential for organizing and accessing complex biological information.
Purpose of the Study:
- To provide a comprehensive and up-to-date resource for restriction enzymes, DNA methyltransferases, and related proteins.
- To detail methylation sensitivity of restriction enzymes and information on R-M systems.
- To make this data accessible through web and FTP interfaces.
Main Methods:
- Data compilation from published and unpublished literature.
- Inclusion of recognition and cleavage sites, isoschizomers, and commercial availability.
- Analysis of R-M systems across over 100 sequenced bacterial and archaeal genomes.
Main Results:
- REBASE contains extensive information on restriction enzymes, DNA methyltransferases, nicking enzymes, and related proteins.
- Detailed data on methylation sensitivity of restriction enzymes is available.
- Information on known and putative R-M systems in numerous microbial genomes is included.
Conclusions:
- REBASE serves as an indispensable, continuously updated resource for researchers in molecular biology, genomics, and biotechnology.
- The database facilitates the study of enzyme specificity, R-M systems, and genome defense mechanisms.
- Accessibility via web and FTP ensures broad utilization by the scientific community.
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