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Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein
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Polymorphisms and the antiviral property of porcine Mx1 protein

Atsushi Asano1, Jae Hong Ko, Takeya Morozumi

  • 1Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

The Journal of Veterinary Medical Science
|January 10, 2003
PubMed

Insights

Pig Mx1 protein provides resistance against vesicular stomatitis virus (VSV). Studies compared Mx1 gene variants from different pig cells, finding both conferred similar antiviral activity against VSV replication.

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Type I interferons induce antiviral proteins, including Mx proteins, crucial for innate immunity.
  • Pig Mx1 protein is an interferon-inducible protein with known antiviral functions.
  • Understanding Mx1 variations is important for studying host-pathogen interactions.

Purpose of the Study:

  • To determine the cDNA sequences of pig Mx1 from PK(15) and LLC-PK1 cells.
  • To compare the antiviral activities of different pig Mx1 protein variants against vesicular stomatitis virus (VSV).

Main Methods:

  • cDNA sequencing of pig Mx1 from PK(15) and LLC-PK1 cell lines.
  • Analysis of Mx1 polymorphisms, including deletions and amino acid substitutions.
  • Transfection of 3T3 cells with Mx1 mRNA and subsequent VSV infection assays.

Main Results:

  • Identified an 11 bp deletion in the 3' coding region of PK(15) derived Mx1 cDNA, leading to amino acid changes and extension compared to LLC-PK1 Mx1.
  • Mx1 mRNA from both PK(15) and LLC-PK1 cells conferred resistance to VSV replication when expressed in 3T3 cells.
  • No significant difference in the efficiency of VSV inhibition was observed between the two Mx1 variants.

Conclusions:

  • Pig Mx1 protein effectively confers resistance to vesicular stomatitis virus (VSV).
  • The identified Mx1 polymorphisms do not significantly alter its antiviral efficacy against VSV in this experimental system.

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