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Gene expression profile in human leukocytes.

Shin-ichi Hashimoto1, Shigenori Nagai, Jun Sese

  • 1Department of Molecular Preventive Medicine, Graduate School of Frontier Science, University of Tokyo, Japan.

Blood
|January 11, 2003
PubMed
Summary
This summary is machine-generated.

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This study provides a comprehensive gene expression database for human leukocytes at resting and activated states using Serial Analysis of Gene Expression (SAGE). It identifies unique gene signatures for different leukocyte types, aiding in understanding immune cell function.

Area of Science:

  • Immunology and Molecular Biology
  • Gene Expression Profiling

Background:

  • Leukocytes, crucial for host defense, are diverse with distinct roles.
  • Understanding their gene expression profiles is vital for defining cell subsets and functions.

Purpose of the Study:

  • To create a comprehensive gene expression database for various human leukocyte types.
  • To identify unique gene expression signatures in resting and activated leukocytes.

Main Methods:

  • Serial Analysis of Gene Expression (SAGE) was performed on 17 libraries of human leukocytes.
  • Analysis included peripheral blood monocytes, macrophages, dendritic cells, granulocytes, NK cells, B cells, and T cells.
  • Gene expression profiles were analyzed using probability and hierarchical clustering.

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Main Results:

  • Over 709,990 SAGE tags were analyzed, identifying 27,323 unique genes expressed in specific leukocyte types.
  • Distinct gene expression patterns were identified for various resting and activated leukocyte subsets.
  • Novel molecular signatures were established for different leukocyte populations.

Conclusions:

  • The study provides a valuable gene expression resource for human leukocytes.
  • Identified gene signatures enhance the molecular definition of leukocyte subsets.
  • This research deepens the understanding of leukocyte biology in host defense.