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A custom-built controlled-rate freezer for small sample cryopreservation studies.

A Medrano1, J Anderson, J D Millar

  • 1Department of Animal Sciences, Facultad de Estudios Superiores - Cuautitlan, National Autonomous University of Mexico 54740 Cuautitlan Izcalli, Mexico. amedrano@servidor.unam.mx

Cryo Letters
|January 11, 2003
PubMed
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A new controlled-rate freezing machine precisely manages sample cooling using a computer-assisted system. This validated device achieves consistent cooling rates up to 80°C/min for biological samples.

Area of Science:

  • Cryobiology
  • Biotechnology
  • Mechanical Engineering

Background:

  • Controlled-rate freezing is crucial for preserving biological samples.
  • Existing methods may lack precision in cooling rate control.
  • Optimizing freezing protocols enhances sample viability.

Purpose of the Study:

  • To describe a custom-built controlled-rate freezing machine.
  • To validate its performance across various cooling rates.
  • To establish an effective freezing protocol for plastic straws.

Main Methods:

  • Utilized a custom-built machine with a stepper motor and thermocouple feedback system.
  • Operated within a static liquid nitrogen vapor column.
  • Implemented a three-phase cooling protocol: +5 to -5°C, nucleation, and -5 to -80°C.

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Main Results:

  • The machine successfully controlled sample cooling rates up to 80°C/min.
  • Demonstrated rapid and consistent absorption of latent heat.
  • Validated the device's performance through experimental testing.

Conclusions:

  • The developed controlled-rate freezing machine offers precise and reproducible cooling.
  • The established protocol is effective for cooling samples in plastic straws.
  • This technology advances cryopreservation techniques for biological materials.