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Related Experiment Videos

Polymer design for corneal epithelial tissue adhesion: pore density.

M D M Evans1, S Taylor, B A Dalton

  • 1CRC for Eye Research and Technology, University of NSW, Sydney, Australia 2052. meg.evans@csiro.au

Journal of Biomedical Materials Research. Part A
|January 11, 2003
PubMed
Summary

Porous polymers with 100-nm pores are crucial for corneal onlays. Optimal pore densities between 0.52% and 14.4% support corneal epithelial tissue adhesion and stratification.

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Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Corneal onlays require porous polymers to support epithelial tissue.
  • Previous research identified 100-nm pores as critical for corneal epithelial cell migration and adhesion.
  • The impact of pore density on tissue response remained to be investigated.

Purpose of the Study:

  • To determine the optimal pore density on polycarbonate surfaces for sustaining corneal epithelial tissue.
  • To evaluate the effect of varying pore densities on epithelial stratification and adhesion structure formation.

Main Methods:

  • Corneal epithelial tissue was cultured for 21 days on polycarbonate with varying pore densities (0.27% to 14.4%).
  • Histology was used to assess epithelial structure.

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  • Electron microscopy quantified the formation of basal lamina and hemidesmosomes at the tissue-polymer interface.

    • Main Results:

    • Epithelial tissue stratified and formed adhesive structures on surfaces with pore densities from 0.52% to 14.4%.
    • Nonporous surfaces and surfaces with very low pore density (0.27%) did not support sustained tissue growth.
    • A maximum of 14.4% surface porosity with 100-nm pores is effective for epithelial tissue maintenance.

    Conclusions:

    • Polycarbonate surfaces with 100-nm pore densities between 0.52% and 14.4% can successfully maintain corneal epithelial tissue.
    • These findings provide critical design parameters for developing advanced implantable contact lenses and corneal onlays.
    • Optimized pore density is essential for achieving functional integration of corneal epithelial tissue with implantable devices.