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Published on: May 23, 2014
Secretion of granulocyte chemotactic protein-2 by cultured human endometrial stromal cells
Shinichiro Mine1, Kaei Nasu, Junichiro Fukuda
1Department of Obstetrics and Gynecology, Oita Medical University, Oita, Japan.
Objective:
To evaluate the expression of granulocyte chemotactic protein-2 (GCP-2) in human endometrial stromal cells.
Design:
The effects of interleukin (IL)-1alpha, IL-1beta, tumor necrosis factor (TNF)-alpha, TNF-beta, interferon (IFN)-gamma, and lipopolysaccharide (LPS) on the production of GCP-2 by endometrial stromal cells were investigated.
Setting:
Research laboratory at a medical university.
Patient(S):
Eight endometrial specimens in the late proliferative phase were used.
Intervention(S):
Endometrial stromal cells were incubated for 24 hours with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, IFN-gamma, and LPS.The concentration of GCP-2 in the culture media was measured using an enzyme-linked immunosorbent assay (ELISA).
Result(S):
A small amount of GCP-2 was detected in the culture media of unstimulated endometrial stromal cells. The production of GCP-2 by endometrial stromal cells was stimulated with IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, and LPS in a dose-dependent manner. Interferon-gamma did not affect GCP-2 production by these cells.
Conclusion(S):
These results suggest that GCP-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells. The modulation of GCP-2 concentrations in the local environment may contribute to the normal and pathological processes of human reproduction by regulating the neutrophil trafficking in the endometrium.
