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Published on: April 8, 2021
[Studies on the metabolism of gentiopicroside by rat intestinal flora]
Objective:
To study the metabolism of gentiopicroside by rat intestinal flora in vitro and time profiles of gentiopicroside and its metabolites in rat serum after oral administration of gentiopicroside.
Method:
Using high performance liquid chromatography (HPLC) for determination of gentiopicroside, gentianine and gentianal content of rat intestinal flora culture medium in vitro and rat serum sample in vivo.
Result:
Gentiopicroside was metabolized quickly in vitro by rat intestinal flora, consequently gentianine and gentianal content appeared. Gentianine and gentianal were observed after gentiopicroside oral administration 4 times in rat serum sample, and gentiopicroside content decreased after oral administration 6 times.
Conclusion:
Gentianine and gentianal were the important bioactive metabolites of gentiopicroside in rats.
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