[Effect of manganese on the ratio of micronucleus cells in mice marrow]
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Updated: Sep 27, 2026
The Micronucleus Assay on Cryopreserved Whole Blood
Published on: February 23, 2024
1Department of Preventive Medicine, Capital University of Medical Sciences, Beijing 100054, China.
Abstract:
The ratio of micronucleus in the bone marrow cell of mice induced by manganese (Mn2+, MnCl2) through intraperitoneal injection at doses of 0, 25, 50 and 100 mg/kg respectively was investigated. The number of micronucleus cells per 1000 polychromatic erythrocyte (PCE) from breast bone marrow was counted. The results showed that micronucleus ratio in bone marrow cells were 12.0 and 16.4 per 1000 in the medium and high dosage groups, which were higher than that of control (P < 0.01). There is no distinct difference between the low dosage group and the control group (P > 0.05), but show a rising trend with the dosage of Mn2+. It indicated that the higher the dose of MnCl2, the stronger the toxic effect to the micronucleus in bone marrow cells. MnCl2 is considered having mutation to chromosome and potential heredity toxicity.
Insights
Manganese (Mn2+) exposure increased micronucleus ratios in mouse bone marrow cells, indicating potential chromosome damage and hereditary toxicity at higher doses. Lower doses showed no significant effect but a rising trend.
Area of Science:
- Toxicology
- Genetics
- Hematology
Context:
- Manganese is an essential trace element, but excessive exposure can lead to neurotoxicity.
- Micronucleus formation is a biomarker for genotoxicity and chromosomal damage.
- Bone marrow cells are sensitive indicators of toxicant effects on cell division and DNA integrity.
Purpose:
- To investigate the genotoxic effects of manganese chloride (MnCl2) on bone marrow cells in mice.
- To determine the dose-dependent relationship between manganese exposure and micronucleus formation.
- To assess the potential for chromosomal mutation and hereditary toxicity induced by manganese.
Summary:
- Mice were administered intraperitoneal injections of manganese chloride (MnCl2) at varying doses (0, 25, 50, 100 mg/kg).
- The ratio of micronuclei per 1000 polychromatic erythrocytes (PCEs) in bone marrow cells was quantified.
- Significant increases in micronucleus ratios were observed at medium (50 mg/kg) and high (100 mg/kg) MnCl2 doses compared to controls (P < 0.01).
- No significant difference was noted at the low dose (25 mg/kg), though a trend of increasing micronuclei with higher Mn2+ dosage was evident.
Impact:
- Results suggest MnCl2 exhibits dose-dependent genotoxicity, inducing chromosomal damage in bone marrow cells.
- The findings highlight the potential for manganese to cause mutations and hereditary toxicity.
- This study contributes to understanding the risks associated with manganese exposure and informs safety guidelines.
