[Genetic transformation of Panax ginseng C.A. Meyer induced by root inducing plasmid(Ri) of Agrobacterium rhizogenes]
1Department of Biotechnology, China Pharmaceutical University, Nanjing 210038, Jiangsu, China.
Objective:
To obtain the hairy root of Panax ginseng (HRPG).
Method:
HRPG was obtained by infecting the germ-free seedling, cotyledon, leaf the blade, Petiole with Agrobacterium rhizogenes 15,834 harbouring agropine-type Ri plasmid. The transformation was proved by PCR and TLC.
Result:
HRPG grew rapidly on hormone-free medium and showed typical phenotype of hairy root. The induced rate and time can be improved by adding exogenous hormone and AS(hydroxyacetosyringone). PCR analysis confirmed the integration of TL-DNA, including 564 bp rolC sequence in the HRPG. Opine analysis evidenced the integration and expression of TL-DNA. The total saponin in HRPG (2.486%) was higher the than control(1.403% Panax ginseng).
Conclusion:
HRPG grows rapidly and proves high in content of ginseng saponin. This culture system of HRPG will be useful for the production of active components in ginseng.
