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Updated: Sep 16, 2026
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
[Effect of liuwei dihuang decoction, on the cytokine expression in splenocytes in AA rats]
1Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Objective:
The effect of Liuwei Dihuang decoction (LW) on the function of splenic T helper cells (Th) was studied and compared with those of the immune inhibitors such as cyclophosphamide (Cy) and cyclosporin A (CsA) in AA rats.
Methods:
The expression of mRNA for IFN-gamma, IL-2, IL-4 and IL-10 in splenic T-Lymphocytes of adjuvant arthritis (AA) rats was evaluated using reverse transcription polymerase chain reaction (RT-PCR) technique.
Results:
mRNA expression level of IL-2 had a tendency to elevate, but the mRNA expression levels of IFN-gamma, IL-4 and IL-10 were significantly decreased in AA rats in comparison with control groups. LW treatment significantly inhibited the mRNA expression of IL-2 and promoted the expression of IFN-gamma, IL-4 and IL-10 in splenocytes of AA rats, with an obvious characteristic as compared with that of Cy or CsA.
Conclusion:
LW can correct the indifferent balance of the functions of splenocyte Th1/Th2 in AA rats.
