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[Preliminary study on apoptosis of human leukemic cells induced by bufalin]
1Medical College of the Chinese People's Armed Police Forces, Tianjin 300162, Tianjin, China.
Objective:
To study the mechanism of inhibiting actions of bufalin on human leukemic cells (HL60).
Methods:
HL60 cells were incubated with bufalin at different concentrations and growth inhibition was analyzed by trypan-blue staining. Cell apoptosis was evaluated by phase contrast microscopy, transmission eletron microscopy and agarose gel electrophoresis.
Results:
Bufalin remarkably inhibited the growth of HL60 cells. Apoptosis of HL60 cells could be effectively induced by bufalin at concentration of 0.01 mumol.L-1 or higher, showing the apoptotic changes in morphology, including cell shrinkage, chromatin agglutination and formation of apoptotic corpuscula; DNA cleavage was observed with agarose gel electrophoresis.
Conclusion:
Bufalin can effectively induce apoptosis of HL60 cells, which is possibly one of the mechanisms for anti-cancer of bufalin.