LytG of Bacillus subtilis is a novel peptidoglycan hydrolase: the major active glucosaminidase
Related Experiment Video
Updated: Sep 27, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
Gavin J Horsburgh1, Abdelmadjid Atrih, Michael P Williamson
1Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom.
Abstract:
LytG (YubE) of Bacillus subtilis is a novel 32 kDa autolysin produced during vegetative growth under the control of Esigma(A) RNA polymerase. Muropeptide analysis of vegetative cells of B. subtilis revealed LytG to be the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Overexpression and purification of LytG allowed its biochemical characterization. Despite sequence homology suggesting muramidase activity, LytG is a novel glucosaminidase with exoenzyme activity and may form part of a novel family of autolysins. It is involved in cell division, lysis, and motility on swarm plates.
More Related Videos
Related Concept Videos
Peptidoglycan Synthesis
Inhibitors of Gram-positive Cell Wall Synthesis

