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Updated: Aug 1, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
[Study on HIV DNA of epidemic HIV-1 strains]
1Department of Infectious Diseases, First Clinical Hospital, Beijing Medical University, Beijing 100034, China.
Objective:
To study the characteristics of variation of HIV DNA in prevalent HIV-1 strains.
Methods:
HIV DNA, extracted from peripheral blood mononuclear cell(PBMC) in 63 patients infected with HIV, was investigated by reverse-transcript PCR (RT-PCR), Long-Distance PCR(LD-PCR), molecular cloning hybridization and sequencing, respectively.
Results:
The subtypes were identified in 57 samples. Subtype B was identified in 43 cases, subtype C in 5 and subtype E in 9. It was found that both full-length and defective HIV DNA in 31 out of 57 cases, defective HIV DNA in 19 cases and full-length HIV DNA in 7 cases. The deleted regions of genome were from 71 to 7,759 nucleotides.
Conclusion:
The variation of gene segments of HIV DNA plays an important role in the infection and epidemic of HIV.
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