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Related Experiment Videos

Human milk contains S100B protein.

Diego Gazzolo1, Giovanni Monego, Valentina Corvino

  • 1Department of Pediatrics, Giannina Gaslini Children's University Hospital, I-16147 Genoa, Italy.

Biochimica Et Biophysica Acta
|January 16, 2003
PubMed
Summary

Researchers discovered the calcium-binding protein S100B and its mRNA in human milk for the first time. Milk concentrations are significantly higher than in other bodily fluids, suggesting a potential trophic role for S100B.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Human Physiology

Background:

  • S100B is a calcium-binding protein found in various tissues.
  • The presence and function of S100B in human milk were previously uncharacterized.
  • Understanding milk composition is crucial for infant development and nutrition.

Purpose of the Study:

  • To investigate the presence and quantify the levels of S100B and its corresponding mRNA in human milk.
  • To compare S100B concentrations in human milk with those in other biological fluids.
  • To explore the potential biological significance of S100B in human milk.

Main Methods:

  • Quantitative immunoluminometric assay for S100B protein detection.
  • Western blot analysis to confirm S100B protein presence.

Related Experiment Videos

  • Reverse transcription-polymerase chain reaction (RT-PCR) with restriction enzyme digestion to detect S100B mRNA.

    • Main Results:

    • S100B protein and its mRNA were unequivocally detected in human milk.
    • Human milk exhibited significantly higher concentrations of S100B compared to cord blood, peripheral blood, urine, cerebrospinal fluid, and amniotic fluid.
    • The findings establish a novel source and higher abundance of S100B in human milk.

    Conclusions:

    • Human milk is a significant source of the calcium-binding protein S100B.
    • The elevated levels of S100B in milk suggest a potential role beyond systemic circulation.
    • A hypothesized trophic function for S100B in neonates warrants further investigation.