Related Experiment Videos
Evaluating the in vitro adhesive strength of biomaterials. Biosimulator for selective leak closure
Roman T Carbon1, Sibylle Baar, Stefanie Kriegelstein
1Department of Paediatric Surgery, Friedrich-Alexander-University Erlangen-Nuremberg, Maximiliansplatz 2, 91054 Erlangen, Germany. roman.carbon@chir.imed.uni-erlangen.de
Aims:
Surgical measures always involve problems of hemostasis and tissue management. Tissue sealing can be carried out in this case, with knowledge of the adhesive strength of liquid and fleece-bound sealing being of great clinical relevance. By employing a leak closure model, it has been possible to perform in vitro standardized technological-biomechanical experiments on a biomembrane mounted in a pressure chamber (biosimulator).
Findings:
Liquid sealing (fibrin gluing: 4.1 hPa, photopolymerisate: 82.9 hPa) offers minimal and maximal adhesive strengths. Fleece-bound sealing does not depend on the carrier material and is always more stabile than pure liquid sealing, whereby systems that must be coated "on the spot" (prepare-to-use: 22.3-25.3 hPa) exhibit significantly lower adhesive strength (p < 0.0001) than a biodegradable collagen system, which is ready-to-use thanks to its fibrinogen-based coating (TachoComb H). Practicability, effectiveness and efficiency are further advantages of ready-to-use systems.
Conclusions:
The biosimulator, presented here, is flexible in terms of its application for tissue management and it enables reproducible, economic and ecological evaluation of repair systems, e.g. tissue sealings.