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Updated: Sep 27, 2026
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
Published on: September 24, 2017
[Effect of hyperthermia on tight junctions between endothelial cells of the blood-brain barrier model in vitro]
Yi-zhao Chen1, Ru-xiang Xu, Qi-jin Huang
1Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Objective:
To investigate the effect of hyperthermia in vitro on tight junctions between the endothelial cells of the blood-brain barrier.
Methods:
An in vitro blood-brain barrier model was established by coculture of ECV304 cells with astrocytes. Transendothelial resistance (TER) of in vitro blood-brain barrier was determined by Millicell-ERS system, and the morphological change of tight junctions examined by silver staining. The expression level of zonula occluden-1(ZO-1) was analyzed by semi-quantitative reverse transcriptase-PCR.
Results:
The integrity of tight junctions was disrupted and the expression level of ZO-1 decreased after treatment with hyperthermia.
Conclusions:
Tight junctions between the endothelial cells of the blood-brain barrier can be destroyed by hyperthermia, and decreased expression level of ZO-1 induced by hyperthermia is one of the most important molecular mechanisms.
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