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[Biological analysis of a novel gene related with interleukin-6]
Rong Xia1, Yan Li, Lun-jiang Lin
1Deparment of Transfusion, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To understand the action mechanism of interleukin (IL)-6 through investigation of its related genes.
Methods:
Using electronic cloning with IL-6-related expressed sequence tags (ESTs), a novel gene at its full length of 924 bp was acquired. Reverse transcriptase-PCR method was subsequently employed to amplify the cDNA of this gene from the total RNA of human U937 cells, which were previously activated with 100 ng/ml IL-6 for 8 h. The fragment was then ligated into the pGEM-Teasy vector and sequence analysis was conducted.
Result:
The whole cDNA gene was successfully fished.
Conclusion:
Electronic cloning technology can be instrumental in identifying novel genes.