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[Application of nitrocellulose membrane in cell culture and pathological techniques]
Hong Zhang1, Ya-dong Wang, Zhuo-sheng Lai
1Surgical Laboratory, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To provide a new supporting medium for carrying out cell culture and histological staining.
Method:
LoVo cell line was cultured using nitrocellulose membrane as the culture medium, in parallel with the same cell culture on the coverglass which served as control.
Results:
The cells cultured on nitrocellulose membrane showed no visible difference from those cultured on coverglasses in terms of cell growth, morphology, and the structure displayed after staining. No toxic effect was observed due to the application of nitrocellulose membrane.
Conclusions:
Nitrocellulose membrane after treatment has such merit as good lucidity and stability without toxicity on the cells. Having also good affinity with the cells, nitrocellulose membrane can offer an promising alternative for glass as cell culture medium.