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Updated: Sep 27, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
[Studies on polysaccharides in different development stages of Armillaria mellea (Vahl.: Fr.) Quel]
1Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100094, China.
Objective:
To study the polysaccharides in different development stages of Armillaria mellea.
Method:
Polysaccharides in rhizomorph, fruit-body, mycelia and its fermenting liquor of A. mellea were extracted, isolated and purified. The properties contents, molar ratio and molecular weight of the polysaccharides were determined by IR spectra, HPLC, GPC and gel chromatography.
Result:
The polysaccharides in mycelia and its fermenting liquor contained only glucose, the sugar contents were 9.00% in mycelia and 0.87 g.(100 ml)-1 in fermenting liquor respectively. The polysaccharides in both rhizomorph and fruit-body consisted of glucose and xylose, and the molar ratio was 1:14 in rhizomorph and 1:10 in fruit-body respectively; the polysaccharide contents were 1.12% in rhizomorph and 2.27% in fruit-body. The molecular weight of these polysaccharides was about 10,000-70,000.
Conclusion:
The experimental results supply important scientific data for developing A. mellea as a medicine.
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