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[Protective effect of tanshinone on injured cultured PC12 cells in vitro]
1Biological & Medicinal Central Laboratory of Anhui, Hefei 230061, Anhui, China.
Objective:
To investigate the protective effects of tanshinone on ischemia-like injury models.
Method:
Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and glutamic acid injury were used to assay the anti-ischemic roles of tanshinone in cultured PC12 cells, by using morphological examination, MTT staining and LDH measurement.
Result:
It was found that 3. 125-200 microliters.ml-1 tanshinone possessed obvious protective effects on PC12 cells from six ischemia-like injury models. Tanshinone could increase the number of life cells and decrease LDH activity significantly, particularly in hypoxia and caffeine injured model.
Conclusion:
Tanshinone protected PC12 cells from all injury models effectively in vitro. Its action may mainly focus on inhibiting development of the primary period of ischemia injury and calcium overloading injury.
