Related Experiment Video
Updated: Sep 27, 2026

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
Immune response to heat shock protein of Helicobacter pylori--a candidate as a vaccine component
Shigeru Kamiya1, Takako Osaki, Haruhiko Taguchi
1Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Mitaka, Japan. skamiya@kyorin-u.ac.jp
Abstract:
. The reactive epitope of heat shock protein 60 (HSP60) of Helicobacter pylori to its monoclonal antibody (H9) was determined, and its synthesized peptide designated pH9 was used for ELISA. The patients with H. pylori infection had significantly lower titers of pH9 antibody than did uninfected patients. In C57BL/6 mice immunized intraperitoneally with the pH9 peptide with Freund's complete adjuvant (FCA), the number of H. pylori organisms colonizing the stomach was significantly lower than that in mice immunized with FCA only. These results suggest that HSP60 of H. pylori is effective in protection against H. pylori infection and might be a good candidate as a vaccine component.
More Related Videos
Related Concept Videos
Gastritis II: Pathophysiology
Other Stress Responses in Bacteria
Peptic Ulcer Disease II: Pathophysiology
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
Peptic Ulcer
Cross-reactivity

