Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative study of methyl-CpG-binding domain proteins.

Tim C Roloff1, H Hilger Ropers, Ulrike A Nuber

  • 1Max-Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany. rolof_t@molgen.mpg.de <rolof_t@molgen.mpg.de>

BMC Genomics
|January 17, 2003
PubMed
Summary

Researchers identified six new methyl-CpG-binding domain (MBD) proteins, expanding the known MBD protein family. These MBD proteins are crucial for gene expression control and understanding them is vital for conditions like Rett syndrome.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computational design of artificial supply networks for engineered human tissue.

Scientific reports·2026
Same author

Guiding the spatial organization of engineered heart tissues.

Advanced drug delivery reviews·2026
Same author

Chromatin remodelling subunit SMARCB1 is implicated in dendrite development and complex brain functions.

Acta neuropathologica communications·2026
Same author

The ATP-mediated cytokine release by macrophages is down-modulated by unconventional α9* nicotinic acetylcholine receptors.

Frontiers in immunology·2025
Same author

Enrichment of FGF8-expressing cells from neurally induced human pluripotent stem cell cultures.

Stem cell reports·2023
Same author

MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation.

Frontiers in cell and developmental biology·2022

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation at CpG sites is a key epigenetic regulator of gene expression in vertebrates.
  • Methyl-CpG-binding domain (MBD) proteins recognize methylated CpGs and are involved in transcription regulation.
  • Five MBD proteins (MBD1-4, MECP2) were previously known.

Purpose of the Study:

  • To identify novel proteins containing methyl-CpG-binding domains.
  • To characterize the MBD sequences, additional protein motifs, and gene expression patterns of newly identified MBD proteins.

Main Methods:

  • Database searches for novel MBD-containing proteins.
  • Sequence analysis and phylogenetic comparison of MBD domains.
  • Northern blot analysis to determine gene expression patterns.

Related Experiment Videos

Main Results:

  • Six new MBD protein sequences were identified, distinct from previously known ones.
  • Phylogenetic analysis revealed at least four distinct MBD protein groups.
  • Two novel proteins, KIAA1461 and KIAA1887, were detected via Northern blot with transcripts of ~8 kb and ~5 kb in all tested human tissues.

Conclusions:

  • A total of eleven MBD polypeptides have been identified in humans and mice.
  • The identified MBD proteins likely play roles in transcriptional regulation.
  • Understanding these MBD proteins and their expression is significant for research into Rett syndrome.