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Updated: Jul 30, 2026

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Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
Published on: September 23, 2015
No hypothermic response to serotonin in 5-HT7 receptor knockout mice
P B Hedlund1, P E Danielson, E A Thomas
1Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Summary
5-hydroxytryptamine (5-HT) causes hypothermia through the 5-HT(7) receptor. Oleamide induces hypothermia via a separate pathway, though it can also interact with the 5-HT(7) receptor.
Area of Science:
- Neuropharmacology
- Receptor pharmacology
Background:
- 5-hydroxytryptamine (5-HT) induced hypothermia was previously attributed to various receptor subtypes.
- Oleamide is a lipid known to induce sleep and hypothermia, and it allosterically interacts with the 5-HT(7) receptor.
Purpose of the Study:
- To investigate the role of the 5-HT(7) receptor in 5-HT induced hypothermia.
- To elucidate the mechanism of oleamide's hypothermic effect in relation to the 5-HT(7) receptor.
Main Methods:
- Utilized 5-HT(7) receptor knockout mice and wild-type mice.
- Administered 5-HT, 5-carboxamidotryptamine, and oleamide to assess hypothermic responses.
- Examined the combined effects of 5-HT and oleamide on body temperature.
Main Results:
- 5-HT failed to induce hypothermia in 5-HT(7) knockout mice, confirming the receptor's role.
- Oleamide effectively induced hypothermia in both knockout and wild-type mice.
- Co-administration of 5-HT and oleamide resulted in additive or synergistic hypothermic effects.
Conclusions:
- 5-HT-induced hypothermia is primarily mediated by the 5-HT(7) receptor.
- Oleamide likely acts through an independent pathway and potentially an allosteric site on the 5-HT(7) receptor to regulate body temperature.

