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Real-time Cytotoxicity Assays in Human Whole Blood
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Real-time Cytotoxicity Assays in Human Whole Blood

Published on: November 7, 2014

Antibody-dependent cell-mediated cytotoxicity in humans. I. Characterization of the effector cell

G Trinchieri, P Bauman, M De Marchi

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1975
    PubMed

    Mononuclear cells from human peripheral blood are able to destroy alloantibody-coated target cells of a human lymphoblastoid line. The effector cell is a nonadherent, non-phagocytic, medium sized lymphoid cell. The cell is not detectable in early human fetuses. Its activity is destroyed by x-ray treatment in vitro but is not inhibited by anti-immunoglobulin sera. Its efficiency in antibody-dependent cell-mediated cytotoxicity is rapidly lost in culture and this loss is prevented by stimulation with allogeneic cells.

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