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Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Published on: October 15, 2018
Nucleotide profile of mouse liver: response to 2'-O-dibutyryl cytidene 3',5'-cyclic monophosphate
Reza Haji Hosseini Baghdad Abadi1
1Department of Biology, Faculty of Science, Payame Noor University, Nakhel Street, Lashkarak Road, Tehran 19569, Iran. hosseini@pnu.ac.ir
Abstract:
The effect of 2'-O-dibutyryl cytidene 3',5'-cyclic monophosphate (dibutyryl cCMP) on the nucleotide profile of mouse liver was examined. Dibutyryl cCMP caused an increase in the amount of CTP in mouse liver. Perchloric acid extracts of liver tissue were neutralized with tri-N-octylamine in trichlorotriflouroethene and, after removal of CLO(4-), subjected to preliminary purification on a Cu2+-loaded column of Chelex 100. A high-pressure liquid chromatographic anion-exchange procedure was used and gave good resolution of the free nucleotides on a single column.

