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Updated: Aug 15, 2026

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
Published on: March 30, 2016
The hepatocyte: heterogeneity and plasticity of liver cells
1Department of Pathology and Laboratory Medicine, Division of Experimental Pathology, MC-151, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208-3479, USA. sells@mail.amc.edu
Abstract:
The heterogeneity of the proliferative response of the liver to injury and during hepatocarcinogenesis implicates at least four levels of cells in the hepatic lineage which respond: the mature unipolar hepatocyte, the unipolar duct cell, a bipolar ductular progenitor cell, and a multipotent periductular liver progenitor cell of presumed extra-hepatic (bone marrow) origin (1). The original concept of "embryonal rests" as the cells or origin of cancer in adult organs (2) may be correct in the sense that progenitor cells in adult tissues that give rise to cancers may derive from circulating pluripotent stem cells.
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