[Pathomorphological features of proliferative vitreoretinopathy in intravitreal administration of blood mononuclear...

The role of blood mononuclears in the pathogenesis of proliferative vitreoretinopathy (PVR) was studied on 25 Wistar rats (200-250 g). Intravitreal injections of 0.05 ml normal saline containing mononuclears (3000/mm@3) into one eye and 0.05 ml normal saline into the other were made through the flat part of the ciliary body under ether narcosis. Mononuclears were isolated using the ficoll-hypaque gradient. Indirect ophthalmoscopy was carried out on days 1, 3, 5, 7, 14, and 21 after injection. After each ophthalmoscopy (except day 1) 5 animals were sacrificed and both eyes were removed. The material was fixed for optic and electron microscopy. Ophthalmoscopic and histological studies showed the development of preretinal adhesions, the main sign of PVR. Bearing in mind the unidirectional flow of liquid in ocular tissues, created by the pressure gradient, the authors suggest that blood mononuclears are involved in the development of vitreoretinal proliferation.