Related Experiment Videos
Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli
Catalina López-Saucedo1, Jorge F Cerna, Nicolas Villegas-Sepulveda
1Dpto. de Biomedicina Molecular, CINVESTAV-IPN, México D.F., México.
Emerging Infectious Diseases
|January 21, 2003
Abstract:
We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.