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Overlapping deletions define novel embryonic lethal loci in the mouse t complex
Hanna H J Chao1, Sarah E Mentzer, John C Schimenti
1Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6445, USA.
Genesis (New York, N.Y. : 2000)
|January 21, 2003
Summary
Researchers identified two critical genes for mouse embryonic development on chromosome 17 using overlapping deletions. These findings highlight the importance of specific genetic loci for embryogenesis and reveal new lethal mutations.
Area of Science:
- Developmental Biology
- Genetics
- Mouse Models
Background:
- The T complex region of mouse chromosome 17 is crucial for embryonic development.
- Identifying genes essential for embryogenesis requires precise mapping of critical loci.
Purpose of the Study:
- To identify and map genes essential for normal mouse embryogenesis.
- To analyze the consequences of nullizygosity in overlapping deletion regions within the T complex.
Main Methods:
- Utilized a set of overlapping deletions (D17Aus9(df10J), D17Aus9(df12J), D17Aus9(df13J), and Del(17)T(7J)) surrounding the D17Aus9 and T loci.
- Examined the effects of combining these deletions to create nullizygous regions.
Main Results:
- Identified at least two functional units within 1 cM of D17Aus9.
- Characterized two new lethal mutations: l17J1 (peri-implantation lethal) and l17J2 (later-acting lethal).
- Del(17)T(7J)/D17Aus9(df12J) embryos exhibited developmental defects including lack of axial rotation, abnormal notochord, and ballooning pericardium, with death around 10.5 days post-coitum (dpc).
Conclusions:
- Overlapping deletion complexes are valuable tools for identifying, mapping, and analyzing genes critical for embryonic development.
- The T complex region harbors multiple essential genes for embryogenesis, with specific loci influencing distinct developmental stages.