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Modified colorimetric method for procainamide in plasma.

D S Sitar, D N Graham, R E Rangno

    Clinical Chemistry
    |March 1, 1976
    PubMed
    Summary
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    A new colorimetric method enhances procainamide (PA) detection in plasma. This improved assay offers twofold sensitivity and a lower detection limit of 0.5 mg/L for procainamide analysis.

    Area of Science:

    • Analytical Chemistry
    • Clinical Chemistry
    • Pharmacology

    Background:

    • Accurate quantification of procainamide in plasma is crucial for therapeutic drug monitoring.
    • Existing colorimetric methods for procainamide determination may lack sufficient sensitivity or have higher detection limits.
    • The need for a more sensitive and precise assay for plasma procainamide analysis is evident.

    Purpose of the Study:

    • To develop and validate a modified colorimetric method for determining procainamide concentrations in plasma.
    • To enhance the sensitivity and lower the limit of detection compared to previously reported methods.
    • To provide a reliable assay for clinical and research applications involving procainamide.

    Main Methods:

    • A modified colorimetric assay was developed utilizing diazotization and coupling reactions.

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  • N-(1-naphthyl)-ethylenediamine dihydrochloride (Marshall's reagent) was employed for the coupling step.
  • The method was applied to the analysis of procainamide in plasma samples.

    • Main Results:

    • The modified colorimetric method demonstrated a twofold increase in sensitivity compared to the original method.
    • The lower limit of detection for procainamide in a 1.0-ml plasma sample was successfully decreased to 0.5 mg/liter.
    • The assay provides enhanced precision and accuracy for procainamide quantification.

    Conclusions:

    • The modified colorimetric method offers a significant improvement for determining procainamide in plasma.
    • The enhanced sensitivity and lower detection limit make the assay suitable for clinical applications.
    • This method provides a valuable tool for monitoring procainamide levels in patients.