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Updated: Sep 27, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
Structural and functional analysis of 3' untranslated region of mouse Period1 mRNA
Shihoko Kojima1, Matsumi Hirose, Katsushi Tokunaga
1Laboratory of Functional Genomics, Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Abstract:
In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3(')-untranslated region (3(')UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3(')UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using LUC::mPer1 3(')UTR fusion genes revealed that the mPer1 3(')UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3(')UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus.
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