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Updated: Jun 22, 2026
Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model
Published on: November 5, 2011
[Cloning and the basic characteristics of a novel cDNA induced by ischemia and reperfusion]
1Central Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To identify and clone genes induced by ischemia and reperfusion.
Methods:
cDNA fragments responding to ischemia and reperfusion in porcine myocardium were isolated by mRNA differential display and then the cDNA library from porcine heart was screened. After cloning, sequencing, and hybridization, the basic characteristics of the gene were analyzed.
Results:
One cDNA fragment from a novel gene up-regulated by ischemia and reperfusion was identified and cloned subsequently; a novel full-length cDNA containing an open reading frame encoding 608 amino acid was obtained by screening the library. Sequencing results revealed that both DNA and amino acid sequences had no substantial homology to previously described DNA or protein sequences. Northern blot analysis confirmed that this gene expression in ischemia and reperfusion myocardium was significantly increased, and the expression was also able to be detected in normal porcine heart, liver, lung, kidney, spleen, intestine, brain, and skeletal muscle. Southern blot analysis clearly showed that this novel gene had high evolutional conservation in human beings, pigs, rabbits, and mice.
Conclusion:
The cloned gene is a novel one induced by ischemia and reperfusion, and it may play a very important role in the response to ischemia and reperfusion.
Insights
Researchers identified and cloned a novel gene significantly upregulated by ischemia and reperfusion. This gene, conserved across species, may play a crucial role in the cardiac response to ischemia.
Area of Science:
- Molecular Biology
- Cardiovascular Research
- Gene Expression Analysis
Context:
- Ischemia and reperfusion injury (IRI) is a critical factor in cardiovascular diseases.
- Understanding the genetic mechanisms underlying IRI is essential for developing therapeutic strategies.
- Porcine myocardium serves as a relevant model for studying human cardiac responses.
Purpose:
- To identify and clone novel genes induced by ischemia and reperfusion (I/R) in the heart.
- To characterize the sequence and expression patterns of a newly discovered I/R-responsive gene.
Summary:
- A novel gene was identified and cloned from porcine myocardium exhibiting increased expression following ischemia and reperfusion.
- The full-length cDNA encodes a 608-amino acid protein with no significant homology to known sequences.
- Northern blot analysis confirmed significant upregulation in I/R myocardium and expression in various normal tissues, while Southern blot indicated evolutionary conservation across mammals.
Impact:
- Discovery of a novel gene involved in the cellular response to cardiac ischemia.
- Provides a new molecular target for investigating and potentially treating ischemia-reperfusion injury.
- Highlights the conserved nature of the response to ischemia across different species, suggesting fundamental biological mechanisms.
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