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[Pilot study on mutations of p53 gene in laryngeal carcinoma]
1Department of Otolaryngology, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Objective:
To determine the characteristic and frequency of the point mutation of p53 gene.
Methods:
22 cases of laryngeal squamous cell carcinoma (LSCC) with positive expression of mutant p53 protein and 2 cases of normal laryngeal epithelium as control were examined by means of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), single strand conformation polymorphism (SSCP) and DNA sequencing analyses.
Results:
3 cases of LSCC had the point mutation on codons 248, 250 and 254 of exon 7 in p53 gene. None of the cases had the point mutation on codon 249.
Conclusion:
These findings suggest that the point mutation on codon 249 of exon 7 in p53 gene may be uncommon in LSCC. SSCP and DNA sequencing analyses are sensitive and rapid methods for detecting p53 gene mutation.