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Related Experiment Videos

[Counting platelets by flow cytometry with platelet-specific antibody CD42a].

L Peng1, H Jiang, H Yang

  • 1Department of Laboratory Medicine, First Affiliated Hospital, WCUMS, Chengdu 610041, China.

Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|January 23, 2003
PubMed
Summary

Flow cytometry (FCM) using CD42a antibodies offers a specific, accurate, and precise method for platelet (PLT) counting. This validated technique shows excellent reproducibility and linearity, making it a strong candidate for a reference PLT count method.

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[Diagnostic value of human cytomegalovirus late-mRNA detection for intrauterine active human cytomegalovirus infection].

Zhonghua fu chan ke za zhi·2001

Area of Science:

  • Hematology
  • Immunology
  • Clinical Chemistry

Context:

  • Accurate platelet (PLT) quantification is crucial for diagnosing and managing various hematological conditions.
  • Existing methods for PLT counting may have limitations in specificity, accuracy, or precision.
  • Flow cytometry (FCM) presents a potential advanced technology for cell counting applications.

Purpose:

  • To evaluate the suitability of flow cytometry (FCM) utilizing CD42a monoclonal antibody as a reference method for platelet (PLT) enumeration.
  • To assess the specificity, accuracy, precision, linearity, and stability of FCM for PLT counting.

Summary:

  • Seventy-six whole blood samples were analyzed using FCM with CD42a antibody, targeting platelet membrane glycoprotein Ib-Ix (GPIb-Ix).
  • PLT counts were determined by multiplying the PLT/RBC ratio from FCM with RBC counts from a calibrated analyzer.

Related Experiment Videos

  • FCM results demonstrated high reproducibility (within-replicate CV < 4%, between-replicate CV < 11%, overall < 5%), excellent linearity (r = 0.9975), and stability within 6 hours at room temperature, with minimal carry-over (0.87%). Comparisons with automated analyzers (SE-9500, NE-1500, CD-1600) and manual counts showed high correlation (r > 0.975).

    • Impact:

    • The study validates FCM with CD42a antibody as a specific, accurate, and precise method for platelet counting.
    • Findings support the adoption of FCM as a candidate reference method for routine platelet enumeration.
    • This advancement could improve the reliability of diagnostic and prognostic assessments reliant on accurate platelet counts.