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[High efficiency gene transfer into breast cancer cells by pseudotyped retroviral vector]
1Department of Oncology, Xiangya Hospital, Central South University, Changsha, 41008.
Objective:
To investigate the efficiency of gene transfer mediated by pseudotyped retroviral vector in breast cancer, and to provide an useful vector for breast cancer gene therapy.
Methods:
We used a MuLV vector pseudotyped with the vesicular stomatitis virus glycoprotein(VSV-G) envelope to transduce breast cancer cells MDA-MB-435, To compare the transfer efficiency with MuLV.
Results:
The transfer efficiency is (92 +/- 12)% by using MuLV/VSV-G vector, (24 +/- 5)% by MuLV vector. The transfer efficiency of MuLV/VSV-G is 3.8 times that of MuLV vector.
Conclusion:
Our results suggest that pseudotyped retroviral vector MuLV/VSV-G is a kind of high efficiency gene transfer vector in breast cancer cells.