[Usefulness of Cofiler PCR amplification kit in parentage testing]

Parentage testing was performed by using multiple PCR and automated genescan and genotype analysis. Parentage was excluded in 36 cases, including 22 of 149 paternity trios and 14 of 37 paternity duos. In the 22 trios and 14 duos in which paternity was excluded, a mean of 6.1 and 5 incompatible STR markers were noted. The application value of six loci which included in the Cofiler amplification kit was showed here. The discrimination power ranged from 0.8023 for TPOX to 0.9203 for D16S539. The cumulative power of exclusion was provided as 0.9825. Three mutation were found (one each at D3S1358, TPOX, CSF1PO). For most nonexcluded paternity duos, only when the Cofiler amplification kit was used in conjunction with Profiler Plus kit, the RCP value was acceptable. The data showed that the Cofiler kit could play an important role in cases of paternity duos.