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Updated: Sep 27, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

CRM1-mediated nuclear export and regulated activity of the Receptor Tyrosine Kinase antagonist YAN require specific

Tina L Tootle1, Philina S Lee, Ilaria Rebay

  • 1Whitehead Institute, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

Development (Cambridge, England)
|January 23, 2003
PubMed

Insights

The study reveals how MAE protein regulates YAN and POINTED activity, crucial for controlling cell growth and preventing cancer by fine-tuning Receptor Tyrosine Kinase (RTK) signaling.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Cancer Research

Background:

  • ETS transcription factors regulate cell processes and are implicated in tumorigenesis when misregulated.
  • YAN acts as a transcriptional repressor, balancing Receptor Tyrosine Kinase (RTK) signaling.
  • Understanding YAN regulation is key to controlling cell proliferation and differentiation.

Purpose of the Study:

  • To elucidate the molecular mechanism of YAN downregulation.
  • To define the role of MAE, a co-factor, in YAN regulation and RTK signaling.
  • To investigate MAE's function in feedback loops controlling ETS factor activity.

Main Methods:

  • Investigated CRM1-mediated nuclear export of YAN.
  • Assessed the role of MAE in YAN phosphorylation and downregulation.
  • Analyzed MAE's impact on POINTED-P2 activation.

Main Results:

  • Demonstrated CRM1-mediated nuclear export as a key mechanism for YAN downregulation.
  • Identified MAE as a crucial co-factor promoting YAN downregulation.
  • Discovered MAE's dual role in YAN regulation and an inhibitory feedback loop on POINTED-P2.

Conclusions:

  • MAE plays multiple, independent roles in modulating YAN and POINTED activity.
  • MAE fine-tunes ETS factor levels in response to RTK signaling.
  • This regulation is critical for preventing inappropriate cellular responses and potential tumorigenesis.

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