Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peptides targeting caspase inhibitors.

Ingo Tamm1, Martin Trepel, Marina Cardó-Vila

  • 1The Burnham Institute, La Jolla, California 97037, USA.

The Journal of Biological Chemistry
|January 23, 2003
PubMed
Summary

Researchers identified novel peptides that specifically target the X-inhibitor of apoptosis protein (XIAP), a key regulator of programmed cell death implicated in cancer. These XIAP-binding peptides show potential for developing new cancer therapies by modulating apoptosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A nationwide mapping of services for high users of emergency departments in England: observational study.

BJPsych bulletin·2026
Same author

Paediatric mental health liaison services in England: findings from a national survey.

BMC health services research·2026
Same author

Wnt signaling is modulated by the buffer-like properties of cadherins.

Molecular biology of the cell·2026
Same author

Conformational ligand-directed targeting of calcium-dependent receptors in acute trauma.

Med (New York, N.Y.)·2025
Same author

Disability justice and collective access to labour and delivery care: a qualitative study.

BMC pregnancy and childbirth·2024
Same author

Impacts of the COVID-19 pandemic on access to sexual and reproductive health services for women and gender-diverse people with disabilities in Canada: a qualitative study.

Sexual and reproductive health matters·2024

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • The X-inhibitor of apoptosis protein (XIAP) is a crucial caspase inhibitor and a member of the inhibitors of apoptosis (IAP) protein family.
  • Overexpression of IAPs, including XIAP, is frequently observed in various cancers and leukemias, correlating with poor patient prognosis.

Purpose of the Study:

  • To identify and characterize novel peptides that specifically bind to XIAP.
  • To explore the potential of these peptides as therapeutic agents for cancer treatment by modulating apoptosis.

Main Methods:

  • Phage display library screening using full-length human XIAP and its baculovirus IAP repeat 2 (BIR2) domain.
  • Surface plasmon resonance (SPR) to determine binding affinity (dissociation constant).
  • Protein-protein interaction assays to validate binding specificity and identify interaction sites.

Related Experiment Videos

Main Results:

  • A consensus peptide motif C(D/E/P)(W/F/Y)-acid/basic-XC was identified through phage display screening.
  • Phage displaying the CEFESC motif specifically bound to the XIAP BIR2 domain with a dissociation constant of 1.8 nm.
  • The identified peptides target a region within XIAP homologous to the executioner caspase-binding loop, and an internalizing peptide (PFKQ) induced programmed cell death in leukemia cells.

Conclusions:

  • Novel peptides targeting XIAP have been successfully identified.
  • These XIAP-binding peptides demonstrate specificity for the BIR2 domain and can induce apoptosis in cancer cells.
  • The identified peptides represent promising prototypes for developing small molecule modulators of apoptosis for cancer therapy.