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Fluoroquinolone resistance in Streptococcus pyogenes.

Sandra S Richter1, Daniel J Diekema, Kris P Heilmann

  • 1Department of Pathology, University of Iowa Healthcare, Iowa City, Iowa 52242, USA. sandra-richter@uiowa.edu

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
|January 23, 2003
PubMed
Summary

High-level fluoroquinolone resistance was found in Streptococcus pyogenes, a bacterium causing infections. Genetic analysis revealed specific mutations in the gyrA and parC genes, contributing to this resistance.

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Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Streptococcus pyogenes is a significant human pathogen.
  • Fluoroquinolones are critical antibiotics for treating bacterial infections.
  • Antibiotic resistance in S. pyogenes poses a growing public health concern.

Observation:

  • A clinical isolate of Streptococcus pyogenes was obtained from blood and urine samples of a 78-year-old female patient.
  • The isolate exhibited high-level resistance to fluoroquinolones, with a levofloxacin minimum inhibitory concentration of 16 microg/mL.

Findings:

  • DNA sequencing identified specific genetic mutations in the isolate.
  • Key mutations included a serine81 to tyrosine substitution in the gyrA gene.
  • Two substitutions were found in the parC gene: serine79 to phenylalanine and alanine121 to valine.

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Implications:

  • These genetic alterations in gyrA and parC are associated with high-level fluoroquinolone resistance in S. pyogenes.
  • This finding represents the second reported case of such resistance in a clinical S. pyogenes isolate.
  • Understanding these resistance mechanisms is crucial for developing effective treatment strategies and monitoring antimicrobial resistance trends.