Related Experiment Video
Updated: Aug 17, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
Quantitative characterization of differential ion suppression on liquid chromatography/atmospheric pressure
1Pfizer Global Research and Development, Groton Laboratories, Groton, CT 06340, USA. Michael_j_avery@groton.pfizer.com
Abstract:
Ion suppression is a known phenomenon in atmospheric pressure ionization mass spectrometry. These suppression effects have been shown to adversely affect the accuracy and precision of quantitative bioanalytical methods. This paper presents a simple procedure for determining the impact of differential ion suppression on bioanalytical methods that utilize atmospheric pressure ionization mass spectrometric (APIMS) detection. This procedure was applied to the assessment of two potential internal standards, and to determine selectivity issues for another analyte which was to be measured in multiple species.
More Related Videos
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
08:10Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry
Published on: July 14, 2017
Related Concept Videos
Chemical Ionization (CI) Mass Spectrometry
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
High-Performance Liquid Chromatography: Types of Detectors