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[Partial gene clone and nif gene homologous sequence analysis of Streptococcus sanguis]
Abstract
Objective:
To analyze the sequence of Streptococcus sanguis chromosome which contains one DNA fragment of 800 base pairs (bp) and discuss Streptococcus sanguis biological features of heredity.
Methods:
Streptococcus DNA of 800-bp genetic fragment was cloned and analyzed by using eukaryotic expression vector.
Results:
By Genbank database, it showed that the 800-bp genetic sequence was highly homologous with other bacterial nifS and nifU gene, and the highest homologous score was 114.
Conclusion:
This nif gene of ATCC 10556 strain may correlate with nutrient metabolism and peroxide hydrogen release of Streptococcus sanguis.