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[Design and synthesizing of human vascular endothelial growth factor (VEGF) peptide]
1Department of Oral and Maxillofacial Surgery, College of Stomatology, West China University of Medical Sciences.
Abstract
Objective:
To predict continuous epitopes of VEGF using synthetic peptide of VEGF as antigen to produce anti-VEGF-peptide monoclonal antibody.
Methods:
According to amino sequence of VEGF189, antigen index program and Goldkey soft were used to predict VEGF epitopes, and the peptide containing one of predicted epitopes was synthesized by 430A Peptide Synthesizer.
Results:
The NH2-terminal 1-26 amino acid sequence of VEGF was determined as synthetic peptide and synthesized.
Conclusion:
The predicted epitope of VEGF was synthesized, which provided an effective method when lacking of VEGF.