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Updated: Sep 27, 2026
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
[The relationship between GPx activity in gingival fluid and clinical parameters of adult periodontitis]
Objective:
To study the relationship of glutathione peroxidase (a natural FR scavenger) in gingival cervical fluid (GCF) of patients with periodontitis.
Methods:
GCF was collected from 44 sites of 23 patients with adult periodontitis. The volume of GCF was measured with Periotron 8000. GCF-GPx activity was determined by DTNB methods before and after treatment, and the periodontal parameters GI, PD and AL were recorded.
Results:
GCF-GPx activity was negatively correlated with PD and AL (P < 0.01, P < 0.005), but was not correlated with GI (P > 0.05). After treatment, clinical parameters decreased while GCF-GPx activity increased (P < 0.001), but the increment of GCF-GPx activity showed no correlation with the base level and the changes of clinical parameters.
Conclusion:
The imbalance between FR's production and scavenging might be an important pathological factor of periodontal diseases, and GCF-GPx might be a promising indicator of periodontal status.