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Updated: Aug 19, 2026
Method of Studying Palatal Fusion using Static Organ Culture
Published on: September 19, 2015
[Significance of programmed cell death in C57BL/6N strain mouse palate process development and cleft palate
1Department of Oral Pathology, Stomatology College, Fourth Military Medical University.
Objective:
To observe the changes of programmed cell death (PCD) in C57BL/6N strain mouse cleft palate formation and study related gene of PCD in future.
Methods:
16 mice (gestation of 10 days, GD10) were divided into the experimental and control groups randomly, then the mice of the experimental group were dosed with retinoic acid(RA) 80 mg/kg, and the tissue slices of the embryo mouse were prepared for TdT-mediated dUTP nick end labeling (TUNEL) staining at GD13(14)(the 14th hour of the 13th day gestation), GD13(22), GD14(8), GD14(14), GD14(22), GD15(8), GD15(22) and GD16(8) respectively.
Results:
In the early development of palate process, the positive index of PCD was significantly higher in the experimental group than in the control group (P < 0.05).
Conclusion:
The effects of RA on mesenchymal cells of the early development of palate process in the experimental group induce PCD, which result in the formation of small palate process and failure of fusing of the shelves.
