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[Effects of TGF beta on BMP-2 gene transfection cells in vitro]
1Department of Oral Pathology, Stomatological College, Fourth Military Medical University.
Objective:
To elucidate the mechanism of bone morphogenetic protein-2(BMP-2) and transforming growth factor beta(TGF beta) on cell differentiation.
Methods:
A phagemid expression vector for human BMP-2 (pBK-B2) was constructed and transfected into the NIH3T3 cells by using lipofectamine. Positive cell clones were selected with G418. The stable transfection and expression of BMP-2 were determined by using in situ hybridization and immunohistochemistry methods. The proliferativity of the transfected cells treated with TGF beta were assayed by MTT method. Alkaline phosphatase (ALP) and osteocalcin (OC) production were also measured.
Results:
The proliferation of the transfected cells was stimulated after being treated with TGF beta (50 ng/ml). But the ALP activity and OC production were inhibited.
Conclusion:
The effects of TGF beta are closely related to many factors, and BMP and TGF beta may be synergistic in cell proliferation and differentiation.